Open Access Pub publishes peer-reviewed, free-to-read open-access articles. Showing
articles matching purification — open any to read the full text,
or download the PDF or XML.
Jul 2020 DOI 10.14302/issn.2377-2549.jndc-20-3460
Nivedharshini TamilvananCorresponding author
Laccase enzyme production is important and more beneficial for environment, because it has many roles like, involved in bioremediation, biodegradation, decolorization of environmental polluted dyes and pharmaceutical sector also. Production of laacse enzyme from bacillus sp as using of Agro waste (r…
Jan 2020 DOI 10.14302/issn.2379-7835.ijn-19-3139
Yaaser Q. AlmulaikyCorresponding author
In this study, a peroxidase from new source was purified using ion exchange and gel filtration techniques. The recovery for peroxidase activity was 19% with 11-fold purification and specific activity of 749 unit/mg protein. Purified peroxidase demonstrated a molecular mass of 39 kDa using gel filtra…
Mar 2019 DOI 10.14302/issn.2471-2140.jaa-19-2699
Hassan M.M. MasoudCorresponding author
Xanthine oxidase is a commercially important enzyme with wide area of medical applications to develop diagnostic kits. Xanthine oxidase was extracted, purified and characterized from sheep liver (SLXO). The purification procedure involved acetone precipitation and chromatography on DEAE-cellulose an…
Dec 2012 DOI 10.14302/issn.2326-0793.jpgr-12-100
Shitao LiCorresponding author
Defining protein-protein interactions is essential for understanding the mechanisms by which cells regulate basic functions, such as metabolism, transcription, and signal transduction. Affinity purification followed by tandem mass spectrometry (AP-MS) has application for discovery of new interactors…
Aug 2023 DOI 10.14302/issn.2639-3166.jar-23-4682
Myunggyu LeeCorresponding author
Objectives The solid-liquid separation (SLS) process generally separates solid and liquid fractions in wastewater and livestock manure. The solid-liquid separation process is an essential pretreatment step for the recycling and purification of pig manure. This study has assessed the separation and/o…
Nov 2021 DOI 10.14302/issn.2690-4837.ijip-21-4015
Caitlin A. Connor DAOM PGDip AMP EHP-CCorresponding author
Copper (Cu) has a strong impact on the function of the immune system through several different pathways. These impacts include helping the function of monocytes, neutrophils, and macrophages, and enhancing Natural Killer cells’ activities. Cu also has a role in antimicrobial properties and inflammat…
Jul 2020 DOI 10.14302/issn.2690-4829.jen-20-3480
Phillip BrummCorresponding author
Conversion of biomass into fermentable sugars is a major requirement for successful and cost-effective biofuels production. The conversion of xylan to sugars requires multiple enzymes including α-glucuronidase. Here we report the cloning, expression, purification and characterization of the α-glucur…
Dec 2018 DOI 10.14302/issn.2377-2549.jndc-18-2430
John P. Richie JrCorresponding author
Introduction: Analysis of 8-oxodG is usually conducted by either chromatography-based methods or by immunochemical methods commonly used based upon their low cost and high-throughput. However, concern regarding the accuracy of ELISA methods has complicated their use. We directly compare the levels o…
Oct 2018 DOI 10.14302/issn.2328-0182.japst-18-2344
Liliana MuschiettiCorresponding author
In recent years, the consumption of dietary supplements (DS) has increased worldwide. In Argentina, approximately 14 million DS units were sold between 2015 and 2017. The adulteration of DS with active pharmaceutical ingredients or their analogues has been reported. This represents an alarming emerg…