Search results for “chromatography”

About 25 results in articles

Open Access Pub publishes peer-reviewed, free-to-read open-access articles. Showing articles matching chromatography — open any to read the full text, or download the PDF or XML.

Liquid Chromatography-Mass Spectrometry (LC-MS) Applications in Food Safety–Review

Dec 2024 DOI 10.14302/issn.2835-2165.jfsh-24-5371
Pradeep SarkateCorresponding author

One of the primary goals and aims of studying chemistry in society is the promotion of the well-being of humanity and the sustainable utilization of the available resources. Chemistry has played a significant role in water treatment, food production, energy production, and medicine, among other crit…

Phytochemical Analysis and Thin Layer Chromatography Profiling of Crude Extracts from Senna Occidentalis(Leaves)

May 2019 DOI 10.14302/issn.2576-6694.jbbs-19-2791
A. M. LawalCorresponding author

Plants used for medicinal practices which were discovered since prehistoric stone ages are termed Medicinal plants, which are also referred to as medicinal herbs, since plants produces bioactive chemical compounds (phytochemicals), this research however, is concerned with the extraction using Soxhle…

Glass Chromatography Application: TLC Separation of Benzoic Esters in Pharmaceutical Products

Dec 2017
Loai AljerfCorresponding author

p-hydroxybenzoic acid esters are used as food and drug preservatives. These compounds were quantised by a reversed-phase thin-layer chromatography method based on the use of silanized silica gel as stationary phase. Thin layers chromatography of silanized silica gel (HF254) is implemented to separat…

Rapid and Sensitive High-Performance Liquid Chromatography/Tandem Mass Spectrometry Method for Determination of Danshensu in Rat Plasma

Nov 2013 DOI 10.14302/issn.2326-0793.jpgr-13-291
Yue-sheng WangCorresponding author

A sensitive, specific and rapid high-performance liquid chromatography/tandem mass spectrometry (hplc–ms/ms) method has been developed and validated for the determination of danshensu (dss) in rat plasma in the present study. The analytes were separated on a c18 column (50 mm×2.1 mm, 1.7 μm) and a t…

Liquid Chromatography Tandem Mass Spectrometry Method for Determination of Febuxostat in Human Plasma to Support A Bioequivalence Study

Mar 2013 DOI 10.14302/issn.2328-0182.japst-12-173
Bhavin N. PatelCorresponding author

A reliable, selective and sensitive liquid chromatography-tandem mass spectrometry (LC-ESI-MS/MS) assay has been proposed for the determination of febuxostat in human plasma using indomethacin as the internal standard (IS). The analyte and IS were extracted from 200 µL of human plasma via liquid-liq…

Chemoenzymatic Synthesis of Purine Modified 3′-deoxy-D-ribonucleosides and Evaluation of Their Antiproliferative Activity

Aug 2026 DOI 10.14302/issn.2377-2549.jndc-26-6392
Grigorii G. SivetsCorresponding author

Chemoenzymatic synthesis of a series of purine modified 3′ deoxyribonucleosides was studied from purine derivatives as acceptors and an excess of 3′-deoxyuridine as the donor, prepared by the chemical deamination of 3′-deoxycytidine, using recombinant uridine and purine nucleoside phosphorylases as…

Synthesis of N-Glycosyl Amides via Hydrolysis of Protected Glycosyl Oxazolines and Ritter-like Reactions of Native Carbohydrates

Jul 2025 DOI 10.14302/issn.2377-2549.jndc-25-5525
Grigorii G. SivetsCorresponding author

A stereoselective synthesis of N-glycosyl amides was studied from available N-glycosyl oxazolines prepared by Ritter-like reactions of protected sugar acetonides. Hydrolysis reactions of the protected pentofuranosyl and hexafuranosyl oxazolines, as precursors of glycosyl amine derivatives, were carr…

Research Gaps in Contaminants of Emerging Concern (CECs): Routes to the Standardization of Chemical Test Methods By GC/LC-Mass Spectrometry: A Review

Jun 2023 DOI 10.14302/issn.2377-2549.jndc-23-4615
Thavrin ManickumCorresponding author

A literature review was undertaken with a focus on 1) identifying the research gaps regarding CECs, 2) identifying the most common ones, and 3) identifying the typical analytical methods/technologies employed, for their analysis. A total of 214 papers were noted, with a total of 21 review articles (…

The Impact of EDTA And Selenite on The Stability of Insulin in Cell Culture Media

Jun 2023
S. Prakasha Gowda A.Corresponding author

Insulin is a frequent peptide hormone addition in serum-free mammalian cell culture media. It contributes in a variety of biological functions, including as promoting cell proliferation, cell cycle progression, and glucose uptake. However, it is unknown how stable insulin is under in vitro cell cult…

Syntheses of Clofarabine and Related C2′-β-fluorinated Nucleoside Analogues

Sep 2022 DOI 10.14302/issn.2377-2549.jndc-22-4277
Grigorii SivetsCorresponding author

A multistep synthesis of 2-chloro-9-(2′-deoxy-2′-fluoro-β-D-arabinofuranosyl) adenine (clofarabine) is described from methyl β-D-ribofuranoside. A new improved method for preparation of 1,2-diacetyl D-ribofuranose derivative was developed via acetolysis of tri-O-pivaloylated D-ribofuranoside and pla…

BaMtox, a Myotoxic Enzyme Isolated from the Venom of the Bothrops Alternatus

Mar 2022 DOI 10.14302/issn.2832-4048.jsm-21-3986
Luiz Fernando Moreira IzidoroCorresponding author

Snake envenomations are responsible for a high percentage of deaths, as these toxic proteins induce severe local and systemic effects. In Brazil, the Bothrops genus is responsible for a satisfactory fraction of accidents, including Bothropsalternatus, recognized as urutu, whose venom is capable of i…

Characterization of Ground Water Quality in Southern and Northern Region of Zanzibar Island

Jun 2021 DOI 10.14302/issn.2377-2549.jndc-21-3740
AJM AbdulCorresponding author Department of Natural Science, School of Natural and Social Sciences, The State University of Zanzibar. P. O. Box 146, Zanzibar-Tanzania.

Millions of people from different countries depend on groundwater, which might contain elevated levels of unhygienic constituents. Poor quality of drinking water in many countries has been forcing people to use bottled water. In the present study, groundwater samples were collected in November 2014…

Study of the Consciousness Energy Healing Treatment and It’s Effect on the Isotopic Abundance Ratio of Ascorbic Acid (Vitamin C)

Apr 2021 DOI 10.14302/issn.2689-2855.jan-21-3771
Snehasis JanaCorresponding author

Ascorbic acid is a water-soluble vitamin (Vitamin C) essential for both the plants and animals for the metabolic process. In this study, the liquid chromatography-mass spectrometry (LC-MS) analytical technique was used to characterize the structural properties and isotopic abundance ratio to evaluat…

Optimization, Production, Purification of Laccase Enzyme from Bacillus sp

Jul 2020 DOI 10.14302/issn.2377-2549.jndc-20-3460
Nivedharshini TamilvananCorresponding author

Laccase enzyme production is important and more beneficial for environment, because it has many roles like, involved in bioremediation, biodegradation, decolorization of environmental polluted dyes and pharmaceutical sector also. Production of laacse enzyme from bacillus sp as using of Agro waste (r…

In Vitro Assessment of Antioxidant Enzymes, Phenolic Contents and Antioxidant Capacity of the Verdolaga (Portulacaceae)

Jan 2020 DOI 10.14302/issn.2379-7835.ijn-19-3144
Yaaser Q. AlmulaikyCorresponding author

In this study, the antioxidants and photosynthetic compounds of Verdolaga were examined. Compounds were extracted from distinctive segments of the verdolaga using various solvents such as methanol (40, 60, 80%), ethanol (40, 60, 80%), acetone (40, 60, 80%), and deionized water. The use of 80% methan…

Study of Organic Pollutants in the Muscles of fish Collected from El-Mahmodia Stream at El-Beheira Governorate, Egypt

Oct 2019 DOI 10.14302/issn.2691-6622.ijar-19-2991
E H RadwanCorresponding author

The purpose of this study is to evaluate the impact of organic pollution of EL-Mahmodia canal on the fish (Oreochromis niloticus)muscles tissues collected from two sites at EL-Mahmodia canal in summer and winter 2017. EL-Mahmodia canal is exposed to excessive of effluents which impact fish. The pres…

Non-Enzymatic Methylglyoxal Formation From glucose Metabolites and Generation of Superoxide Anion Radical During Methylglyoxal-Dependent Cross-Links Reaction

Sep 2019 DOI 10.14302/issn.2471-2140.jaa-19-2997
K.B. ShumaevCorresponding author

The paper explores the formation of a-oxoaldehydes during the interaction of glucose metabolites with hydroxyl or alkoxyl radicals. Hydroxyl radicals were generated under radiolysis of aqueous solutions, and alkoxyl radicals (t-BuO) were obtained in the model system tert-butyl hydroperoxide/Fe2+. H…

Antibacterial and Cytotoxicity Activities of Major Compounds from Tinospora cordifolia Willd. Growing on Mangifera indica L.

Apr 2019 DOI 10.14302/issn.2379-7835.ijn-19-2690
Thongchai TaechowisanCorresponding author

Objective To investigate the major constituents of Tinosporacordifolia Willd. growing on Mangiferaindica, and to evaluate the efficacy of their antibacterial and cytotoxicity activities. Methods The ethanolic stem extract of T. cordifolia was subjected to silica gel 60 column chromatography, thin la…

Purification and Characterization of Xanthine Oxidase from Liver of the Sheep (Ovis Aries)

Mar 2019 DOI 10.14302/issn.2471-2140.jaa-19-2699
Hassan M.M. MasoudCorresponding author

Xanthine oxidase is a commercially important enzyme with wide area of medical applications to develop diagnostic kits. Xanthine oxidase was extracted, purified and characterized from sheep liver (SLXO). The purification procedure involved acetone precipitation and chromatography on DEAE-cellulose an…

Comparison of an HPLC-MS/MS Method with Multiple Commercial ELISA Kits on the Determination of Levels of 8-oxo-7,8-Dihydro-2'-Deoxyguanosine in Human Urine

Dec 2018 DOI 10.14302/issn.2377-2549.jndc-18-2430
John P. Richie JrCorresponding author

Introduction: Analysis of 8-oxodG is usually conducted by either chromatography-based methods or by immunochemical methods commonly used based upon their low cost and high-throughput. However, concern regarding the accuracy of ELISA methods has complicated their use. We directly compare the levels o…

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