Search results for “Substrate”

About 19 results in articles

Open Access Pub publishes peer-reviewed, free-to-read open-access articles. Showing articles matching Substrate — open any to read the full text, or download the PDF or XML.

Assessing Performance of Cattle Dung and Waste Cooked Foods in Producing Biogas as Single Substrate and Mixed Substrates in Kampala Uganda

Apr 2019 DOI 10.14302/issn.2377-2549.jndc-19-2701
J Mutesasira,Corresponding author External studies department, Institute of distance Education, Makerere University

Biogas is anaerobic degradation product formed from aqueous slurry of organic waste in a digester. It can be produced from cattle dung,(cd)chicken droppings, decaying leaves, kitchen waste foods(kwf), sewage sludge, slaughter house, goat, pig or sheep manure, Aqueous slurry of 200g/L of mixed or sin…

Lignocellulosic Waste as a Sole Substrate for Production of Crude Cellulase from Bacillus subtilis PJK6 Under Solid State Fermentation Using Statistical Approach

Dec 2018
Nishi K. BhardwajCorresponding author

The agricultural organic wastes pose major environmental issues and their inappropriate disposal is a major cause of pollution while these as cheap lignocellulosic resources can be utilized for many value added products such as enzymes. The purpose of the present study was to utilize wheat bran as a…

Substrate Stiffness Influences the Time Dependence of CTGF Protein Expression in Müller Cells

Jan 2018 DOI 10.14302/issn.2578-8590.ipj-17-1910
William J FosterCorresponding author

Following ocular trauma and retinal detachment, gliotic changes in the retina may develop over the subsequent month, a process known as PVR (proliferative vitreoretinopathy). There have been no successful therapeutic interventions to inhibit PVR. The protein CTGF (Connective Tissue Growth Factor) ha…

Benzoate Concentration and Cooperativity by a Substrate for Benzoate 1,2-dioxygenase from Benzoate-Degrading Rhodococcus Opacus 1CP

Dec 2017 DOI 10.14302/issn.2576-6694.jbbs-17-1860
Inna P. SolyanikovaCorresponding author

We explored the effect of a change in substrate-benzoate (as sole carbon and energy source) concentration in growth medium on the activity of benzoate 1,2-dioxygenase (BDO) of R.opacus 1CP cells, where BDO is the enzyme mediated the initial attack of benzoate. The activity of the enzyme was estimate…

Correlation of Oncotype DX Recurrence Score with the Expression of Insulin Receptor Substrate Proteins in Estrogen Receptor + Breast Cancer

May 2014 DOI 10.14302/issn.2572-3030.jcgb-13-369
Ashraf KhanCorresponding author

Insulin receptor substrate (IRS) 1 and 2 are downstream signaling proteins that influence breast pathophysiology. IRS-1 promotes carcinoma cell proliferation; whereas IRS-2 regulates cell motility, invasion, and glycolysis. Our lab has shown that distinct cellular localization of IRS-2 also plays a…

Chemoenzymatic Synthesis of Purine Modified 3′-deoxy-D-ribonucleosides and Evaluation of Their Antiproliferative Activity

Aug 2026 DOI 10.14302/issn.2377-2549.jndc-26-6392
Grigorii G. SivetsCorresponding author

Chemoenzymatic synthesis of a series of purine modified 3′ deoxyribonucleosides was studied from purine derivatives as acceptors and an excess of 3′-deoxyuridine as the donor, prepared by the chemical deamination of 3′-deoxycytidine, using recombinant uridine and purine nucleoside phosphorylases as…

Keratinophilic Fungi: Diversity And Abundance in the Soil of Ajmer District, Rajasthan

Jul 2025 DOI 10.14302/issn.2766-869X.jfd-25-3841
Renu JangidCorresponding author

Soil is a natural habitat and ecosystem for microorganisms, including bacteria and fungi. Mostly keratinophilic fungi are found in soil, use keratin as a growth substrate, and are essential to the natural degradation of keratin waste. These fungi include dermatophytes, a potential source of infectio…

Adopting a Wider Approach for Fisheries Management

May 2022 DOI 10.14302/issn.2643-0282.imsj-22-4160
Anthony Donald HawkinsCorresponding author

Many marine and freshwater fisheries are now in a very poor state, and many fish stocks are close to collapse. There is a need to manage fisheries more carefully, especially since fishing can affect other aspects of the environment. It is also important to reduce the adverse effects of climate chang…

Thin Film Deposition and Characterization Techniques

Apr 2022 DOI 10.14302/issn.2831-8846.j3dpa-22-4066
Temesgen GeremewCorresponding author

Thin films are everywhere in the modern world, with many of the technologies we depend upon in daily life being, in turn, dependent upon thin film technology. Chemical bath deposition includes principles of chemical bath deposition (CBD) and concept of solubility product, nucleation and film growth,…

A Review on Lab Scale Cultivation of Calocybe Indica and Its Medicinal Value

Dec 2020 DOI 10.14302/issn.2379-7835.ijn-20-3406
Priya PetchimuthuCorresponding author

Calocybe indica, a tropical edible mushroom and it is popular because it has good nutritive value and it can be cultivated commercially on a large scale. Mushrooms are in the great demand everywhere and hold a unique place in the world today due to their typical taste and rich in protein, vitamins,…

Optimization, Production, Purification of Laccase Enzyme from Bacillus sp

Jul 2020 DOI 10.14302/issn.2377-2549.jndc-20-3460
Nivedharshini TamilvananCorresponding author

Laccase enzyme production is important and more beneficial for environment, because it has many roles like, involved in bioremediation, biodegradation, decolorization of environmental polluted dyes and pharmaceutical sector also. Production of laacse enzyme from bacillus sp as using of Agro waste (r…

Arbuscular Mycorrhizal Biofertilizers Sources in the Potato (Solanum Tuberosum) Plant show Interactions with Cultivars on Yield and Litter-bags Spectral Features

Feb 2020 DOI 10.14302/issn.2639-3166.jar-20-3185
Giorgio MasoeroCorresponding author

Four strains of Arbuscular Mycorrhizal (AM) biofertilizer fungi, combined with two potato cultivars, were in-field tested in a four-replicate arrangement in a factorial experiment. As far as general combinability is concerned, cv. Agria was more responsive to different inocula (yield +5.56%, P 0.02)…

Peroxidase from Coleus Forskohlii: Purification and Biochemical Characterization

Jan 2020 DOI 10.14302/issn.2379-7835.ijn-19-3139
Yaaser Q. AlmulaikyCorresponding author

In this study, a peroxidase from new source was purified using ion exchange and gel filtration techniques. The recovery for peroxidase activity was 19% with 11-fold purification and specific activity of 749 unit/mg protein. Purified peroxidase demonstrated a molecular mass of 39 kDa using gel filtra…

Journal of Enzymes Open Access

Trichoderma reesei (Hypocrea jecorina) Bgl1 is a Novel, Exo-Acting Glucanase and Xylanase

Dec 2019 DOI 10.14302/issn.2690-4829.jen-19-3105
Phillip BrummCorresponding author

Trichoderma reeseiβ-glucosidase (Bgl1) is one of four enzymes demonstrated to act synergistically to degrade cellulose both in vitro and in vivo. Our work attempted to better understand the substrate specificity and potential biotechnological applications of Bgl1. T. reesei Bgl1H cleaves over 80% of…

Graphical Approach to Compare Concentration Constants of Hill and Michaelis-Menten Equations

Sep 2018 DOI 10.14302/issn.2576-6694.jbbs-18-2280
Elena V. EmelyanovaCorresponding author

The aim of present study was to describe the graphical technique how to go from Hill concentration constant to Michaelis constant. To compare enzymatic processes, the kinetics of which is subjected to different regularities, it is possible to use constants that characterize catalytic activity (Vmax)…

Polysaccharide Transglycosylases: A Survey of Assay Methods

May 2018 DOI 10.14302/issn.2832-5311.jpcd-18-2077
Vladimír FarkašCorresponding author

Polysaccharide transglycosylases (PTGs) are a unique group of glycoside hydrolases playing important roles in the formation and modification of plant and fungal cell walls. Their action involves cutting the molecule of the polysaccharide substrate at the glycosidic bond, followed by transfer of the…

Journal of Enzymes Open Access

Hydrolysis of Cellulose by Soluble Clostridium Thermocellum and Acidothermus Cellulolyticus Cellulases

Apr 2018 DOI 10.14302/issn.2690-4829.jen-18-2025
Phillip BrummCorresponding author

The goal of this work was to clone, express, characterize and assemble a set of soluble thermostablecellulases capable of significantly degrading cellulose. We successfully cloned, expressed, and purified eleven Clostridium thermocellum (Cthe) cellulases and eight Acidothermuscellulolyticus(Acel) ce…

Isolation and Screening of Phb Producing Halotolerant Bacterial Strains from A Saline Environment

Nov 2017 DOI 10.14302/issn.2576-6694.jbbs-17-1783
VeenaGayathri KrishnaswamyCorresponding author

Plastic materials have been used as packaging materials and also have other numerous applications because of their durability and stability. Plastic products are non degradable and they continue to exist in the environment thereby causing a serious threat to the ecosystem. Bioplastics which are biod…

Kynurenines and Vitamin B6: Link Between Diabetes and Depression.

Sep 2013 DOI 10.14302/issn.2374-9431.jbd-13-218
Gregory OxenkrugCorresponding author

The increased association between depression and diabetes mellitus is generally acknowledged. Recent studies suggest that depression leads to diabetes.However, the underlying molecular mechanisms for this association remain unclear.Literature and our data indicate that inflammatory and/or stress fac…

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